大鼠血栓素B2(TXB2)試劑盒
實(shí)驗(yàn)原理：
本試劑盒用于測(cè)定大鼠血清，血漿及相關(guān)液體樣本中血栓素B2(TXB2)的含量。應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中大鼠血栓素B2(TXB2)水平。用純化的大鼠血栓素B2(TXB2)抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入血栓素B2(TXB2)，再與HRP標(biāo)記的血栓素B2(TXB2)抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的血栓素B2(TXB2)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計(jì)算樣品中大鼠血栓素B2(TXB2)濃度。
Rat Thromboxane B2 (TXB2) ELISA Kit.
Principle of the assay
The kit assay Rat TXB2 level in the sample，use Purified Rat TXB2 antibody to coat microtiter plate wells, make solid-phase antibody, then add TXB2 to wells, Combined TXB2 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TXB2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
上海華壹生物科技有限公司
Shanghai HuaYi Bio-technology Co.,Ltd
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