*調(diào)節(jié)*核苷酸交換因子1抗體,Anti-Epac1抗體

研究領(lǐng)域 免疫學(xué)  神經(jīng)生物學(xué)  信號轉(zhuǎn)導(dǎo)  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human, Mouse, Rat, Rabbit, 
產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 99kDa
細(xì)胞定位 細(xì)胞膜 
性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human Epac1
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
訂購:或（成）客服

英文名稱 Epac1
抗體名稱 *調(diào)節(jié)*核苷酸交換因子1抗體,Anti-Epac1抗體
別    名 bcm910; CAMP GEFI; cAMP regulated guanine nucleotide exchange factor I; CAMPGEFI; CGEF 1; CGEF1; EPA1; Epac 1; EPAC; EPAC1; Exchange factor directly activated by cAMP 1; Exchange protein directly activated by cAMP 1; MGC21410; RAP guanine nucleotide exchange factor; Rap guanine nucleotide exchange factor (GEF) 3; RAP guanine nucleotide exchange factor 3; Rap1 guanine nucleotide exchange factor directly activated by cAMP; RAPGEF3.  

濃 度 1mg/1ml 
規(guī) 格 0.1ml/0.2ml(100ug/200ug) 
公司提供的抗體達(dá)一萬多種,公司擁有Complexin I/復(fù)合素1抗體一抗,二抗,標(biāo)記一抗,標(biāo)記二抗等抗體種類,*價供應(yīng)。
多克隆抗體制備服務(wù)：應(yīng)用保證型抗體制備（ELISA,Western Blot,IHC）和修飾特異性抗體制備（磷酸化,甲基化,乙?；?。
單克隆抗體制備服務(wù)：應(yīng)用保證型抗體制備（ELISA,Western Blot,IHC）和修飾特異性抗體制備（磷酸化,甲基化,乙?；?。
抗體純化服務(wù)：Protein A/G/L純化,抗原親和純化。
zui終抗體質(zhì)檢：成品抗體或抗血清,必須通過客戶要求的所有驗證（ELISA,Western Blot,IHC等）。所有的抗原和抗體驗證數(shù)據(jù)提交客戶。
免疫組化的呈色深淺可反映抗原存在的數(shù)量，可作為定性、定位和定量的依據(jù)。
1.陽性細(xì)胞染色分布有三種類型：細(xì)胞漿、細(xì)胞核、細(xì)胞膜表面。大部分抗原見于細(xì)胞漿，可見于整個胞漿或部分胞漿
2.陽性細(xì)胞分布可分為灶型和彌漫性
3.由于細(xì)胞內(nèi)含抗原量不同，所以染色強(qiáng)度不一。如果細(xì)胞之間染色強(qiáng)度相同，常提示其反應(yīng)為非特異性
4.陽性細(xì)胞染色定位于單個細(xì)胞，且與陰性細(xì)胞相互交雜分布；而非特異性染色常不限于單個細(xì)胞，而是累及一片細(xì)胞。
5.切片邊緣、刀痕或褶皺區(qū)域，壞死或擠壓的細(xì)胞區(qū)，膠原結(jié)締組織等，常表現(xiàn)為相同的陽性染色強(qiáng)度，不能用于判斷陽性。

產(chǎn)品介紹 The activation of RaP1 by cAMP is independent of PKA and is mediated by recently discovered family of guanine nucleotide exchange factors (GEFs) called cAMP-GEFs or Epacs. The Epac signaling therefore represents a novel mechanism for cAMP signaling with in the cAMP cascade. There are 2 members of the Epac family, Epac1 and Epac 2. Both proteins are multidomain proteins containing an autoinhibitory cAMP-binding domain that inhibits the catalytic region and a DEP domain (dishevelled, Egl-10 and pleckstrin homology domain) targeting the membrane anchors. EPAC2 has an additional cAMP-binding site in its N-terminus that binds cAMP with low affinity. EPAC1 mRNA is broadly expressed, with particularly high levels occurring in the thyroid, ovary, kidney and certain brain regions, whereas expression of EPAC2 mRNA appears to be restricted to the brain and adrenal glands. Epac 1 and Epac 2 also interact with light chain 2 (LC2) or MAP1A that serves as a scaffolding structure to stabilize the signal transduction complex. The Epac 1-selective antibodies were generated against unique antigenic sequences form near N-terminus and between RasGEFN and Ras GEF domains. The antibodies to Epac 1are affinity purified over immobilized antigen based chromatography.
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